Diagnostic Swabs in Pertussis

Recovery of Bordetella pertussis from nasopharyngeal swabs.

Isolation of Bordetella pertussis from swabs.

Four varieties of swabs held in plastic containers and three swab transport media kits, all obtainable in the United Kingdom, were tested to assess their effectiveness in the isolation of Bordetella pertussis. The highest isolation rates up to 48 hours were obtained with Dacron swabs held in plastic containers. Swab-transport media kits proved unsatisfactory. B pertussis may have a stronger aff...

The diagnosis of pertussis with supralaryngeal and modified pernasal swabs.

This paper analyses the results of diagnostic swabs taken from children seen at a pertussis contact clinic between December, 1950, and April, 1952. Two new diagnostic techniques entailing contaminating swabs by cough, which were developed during this period, yielded a high percentage of positive cultures. A preliminary communication on one of these new techniques was published when the initial ...

Comparison of nasopharyngeal aspirates with swabs for culture of Bordetella pertussis.

Nasopharyngeal samples were collected from 117 children by aspiration from one nostril and by swab from the other one and cultured for Bordetella pertussis. Among 33 culture-positive specimens, there were 30 positive aspirates and 26 positive swab specimens. Aspirates are easily divided and saved for investigations with other assays which may further improve diagnostic sensitivity. As the aspir...

Regulation of pertussis toxin and lipopolysaccharide levels of Bordetella pertussis 134 in response to modulators

  Whooping cough (pertussis) is a highly contagious disease of the human respiratory tract, which is caused by Bordetella pertussis. Reemerge of pertussis in some highly immunized populations and divergency in gene order among several B. pertussis strains promoted this research to study the change of pertussis toxin (PT) and lipopolysacharide levels in response to the different environments. Th...